Aflatoxin Related p53 Codon 249 Point Mutation in Korean Hepatocellular Carcinoma.
- Author:
Hee Jung WANG
1
;
Seong Woo HONG
;
Young Seok PARK
;
Wook Hwan KIM
;
Myung Wook KIM
Author Information
1. Department of surgery, Ajou University School of Medicine, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
Hepatocellular carcinoma;
p53;
codon 249;
Aflatoxin,
- MeSH:
Aflatoxins*;
Axons;
Carcinoma, Hepatocellular*;
Codon*;
Digestion;
DNA;
Endopeptidase K;
Genes, p53;
Humans;
Korea;
Point Mutation*;
Polymerase Chain Reaction
- From:Korean Journal of Hepato-Biliary-Pancreatic Surgery
1998;2(1):79-83
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: P53 point mutation, especially G to T transversion at codon 249, has been found in many cases of the aflatoxin-related hepatocellular carcinoma(HCC). This study was aimed to exam the mutations of the third base at codon 249 of the p53 gene in Korean HCC. MATERIALS AND METHODS: Forty two patients with resectable HCC were enrolled. Genomic DNA was prepared by proteinase K digestion and phenol/chloroform extractions. A part of DNA including axon 7 of p53 was amplified by PCR. Restriction fragment polymorphism (RFLP) was analyzed after treatment of Hae III restriction enzyme. And the point mutation were confirmed using the direct sequencing method. RESULTS: P53 point mutation, G to T transversion at codon 249 was observed in only one tissue specimen(2.4%). CONCLUSION: P53 point mutation at codon 249 does not correlate with HCC in Korea.
