The mutation characteristics of drug-resistance of Mycobacterium tuberculosis in Qinzhou,Guangxi
10.3969/j.issn.1673-4130.2018.08.011
- VernacularTitle:广西钦州地区结核分枝杆菌相关耐药基因变异特征分析
- Author:
Chongjian LI
1
;
Jingui BAO
;
Zhuobin CHEN
;
Shijuan LI
;
Haisong WU
;
Ling LIANG
Author Information
1. 广西医科大学第十附属医院暨钦州市第一人民医院检验科
- Keywords:
Mycobacterium tuberculosis;
drug-resistance;
gene mutation;
reverse dot blot hybridiza-tion technique construct specific PCR reaction system
- From:
International Journal of Laboratory Medicine
2018;39(8):936-938
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the variation characteristics of rpoB,katG,inhA,rpsL and embB related genes of Mycobacterium tuberculosis(MTB)in Qinzhou,Guangxi.Methods PCR reverse point hybridization was used to detect 5 common resistance mutants of Mycobacterium tuberculosis in 237 MTB-DNA positive sputum samples.Results Among 237 cases of tuberculosis patients,72 cases(30.38%)were resistant to the four kinds of anti-TB drugs.The resistance mutation rate of rifampin,isoniazid and streptomycin was 2.53%, 13.92%,3.80%.The top 5 gene mutation detection loci of Mycobacterium tuberculosis were-15M,S531L and 43M.Conclusion The main drug-resistant strains are isoniazid resistance,and the mutation of inhA gene were the major one in Qinzhou,Guangxi.
