The mechanism of metformin induced human choriocarcinoma cell line JAR apoptosis
10.3969/j.issn.1673-4130.2018.07.027
- VernacularTitle:二甲双胍诱导人胎盘绒毛癌细胞系JAR凋亡的机制初探
- Author:
Guangfen LIU
1
;
Chunyan YAO
Author Information
1. 武警重庆市消防总队医院检验科
- Keywords:
human choriocarcinoma cell line;
metformin;
cell apoptosis;
AMPK/mTOR
- From:
International Journal of Laboratory Medicine
2018;39(7):859-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of metformin on apoptosis of human chorio-carcinoma cell.Methods The human choriocarcinoma cell line JAR was selected and divided into control and metformin groups(final concentrations were 5,10,20,40 and 80 mmol/L).Confocal microscope was adopted to detect cell apoptosis after 48 h treatment.The mRNA and protein expression of AMPK,p-AMPK,mTOR, Caspase-3,Bcl-2 and Bax were measured by Real-time PCR and Western blot respectively.Results Compared with the control group,apoptosis rate of JAR cells in the metformin group(final concentration was 40 mmol/L)was remarkably increased.Metformin activated AMPK by phosphorylation and inhibited mTOR pro-tein expression,meanwhile mRNA and protein expression levels of Caspase-3 and Bax were significantly in-creased,but Bcl-2 mRNA and protein expression were significantly decreased.Conclusion Metformin induces the JAR cells to generate apoptosis by the AMPK/mTOR pathways and Caspase-3,Bcl-2/Bax pathways simaltaneously.
