The application of SPR technology in prenatal testing of fetal RHD blood type gene
10.3969/j.issn.1673-4130.2018.07.006
- VernacularTitle:SPR技术在产前检测胎儿RhD血型基因中的应用
- Author:
Miaoshan LI
1
;
Changlin WU
;
Jian'an HE
;
Dayong GU
;
Chaopeng SHAO
Author Information
1. 广东省梅州市中医医院检验科
- Keywords:
RhD blood gene;
preenee detecion;
HDN;
SPR technology
- From:
International Journal of Laboratory Medicine
2018;39(7):788-791
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the feasibility of detecting fetus RhD type gene by Surface Plasmon Reso-nance(SPR)technology,and to establish a new rapid diagnosis method for fetus RhD type gene.Methods The different types of DNA corresponding RNA probes were fixed on the surface of SPR chip by using amino cou-pling methods,and optimize the chip analysis condition,and then using the RNase H enzyme hydrolysis,signal amplification detection,at last the detection conditions were determined.We use the RhD type gene exon 5,7 of RNA probe to test its corresponding DNA molecules,and analyse the specificity and sensitivity of SPR chip detection signal.Results The SPR technique for detecting the exon 5,7 of RhD blood type gene shows good sensitivity and specificity in all,SPR technology can specifically detect the Exon 5,7 of RhD blood type gene, and the sensitivity of for detecting RhD gene exon 5,7 is 100 pmol/L by SPR.Conclusion The SPR technolo-gy can quickly detect RhD gene accordingly,SPR technology is simple,rapid,reliable and label-free,w hich can provide a new way predicting fetal RhD type for RhD negative prenatal.
