The application of MALDI-TOF MS in gene polymorphism of clopidogrel
10.3969/j.issn.1673-4130.2018.05.008
- VernacularTitle:MALDI-TOF MS在氯吡格雷基因多态性检测中的应用
- Author:
Qing DENG
1
;
Yuanxue YI
Author Information
1. 重庆医科大学
- Keywords:
MALDI-TOF MS;
clopidogrel;
gene polymorphism
- From:
International Journal of Laboratory Medicine
2018;39(5):543-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of gene polymorphism with clopidogrel and the application value of matrix assisted laser resolution ionization flight time mass spectrometry(MALDL-TOF MS) in gene polymorphism of clopidogrel.Methods A total of 160 cases of inpatient of Armed Police Force Hospitals with acute coronary syndrome (ACS) prepared for treated with percutaneous coronary intervention (PCI) from 2015 to 2017 were selected,platelet aggregation rate at fifth days after clopidogrel antiplatelet therapy was determined and gene polymorphism of CYP2C19 * 2 *,* 3,* 4,* 5,* 17 and ABCB1,CES1,PON1 were analyzed by matrix assisted laser resolution ionization flight time mass spectrometry.Results All the inpatients were divided into the clopidogrel resistance(CR) group(n=75) and NCR group(n=85) according to the maximum aggregation rate of platelets (MAR).The detection rate of the 160 samples was 100％;neither group found CYP2C19 * 4,CYP2C19 * 5,CYP2C19 * 17mutations;the risk of clopidogrel resistance in CYP2C19 * 2 allele A and * 1/* 2,* 1/* 3,* 2/* 2,* 2/* 3,ABCB1 allele T and PON1 allele T were high,and the risk of clopidogrel resistance in CES1 allele T was low,all the differences were statistically significant (P＜0.05).There was a high risk of clopidogrel resistance in CYP2C19 * 3 allele A and a low risk in CYP2C19 * 3/* 3,but there were no statistically significant differences (P＞0.05).Conclusion CYP2C19 * 2,* 3,ABCBI and PON1 gene mutation increases the risk of clopidogrel resistance;CES1 mutation reduces the risk of clopidogrel resistance;MALDI-TOF MS has the advantages of high accuracy,large flux,high speed,low cost,which is suitable for the detection of polymorphism of clopidogrel gene and helpful in guiding rational and individualized medicine.
