Application of MALDI-TOF MS for identification of common bacteria in clinical microbiological test
10.3969/j.issn.1673-4130.2018.05.006
- VernacularTitle:MALDI-TOF MS用于临床微生物检验中常见菌鉴定的应用探讨
- Author:
Lili ZHANG
1
;
Shouyu ZHOU
;
Shipeng CHEN
;
Sen QIAO
Author Information
1. 遵义医学院医学检验系
- Keywords:
MALDI-TOF MS;
microorganism identification;
isolated microorganisms
- From:
International Journal of Laboratory Medicine
2018;39(5):534-536,542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application value of MALDI-TOF MS system in clinical routine separation microorganism by comparing two kinds of microorganism identification system MALDI-TOF MS and Vitek 2 Compact.Methods The bacterial species database of MALDI-TOF MS system and the Vitek 2 Compact system were compared,the new strains from MALDI-TOF MS library were screened out,and the number and frequency of new strains detected from May 2015 when MALDI-TOF MS was put in use to December 2016,and the frequency of detection of common pathogenic bacteria with high confidence were counted.Results There were 205 more new strains in the MALDI-TOF MS library,compared with Vitek 2 Compact.From May 2015 to December 2016,206 times were detected in clinical microbiological examination,and 286 cases if 4 kinds of clinical common pathogenic bacteria were identified by MALDI-TOF MS system with high confidence.Conclusion MALDI-TOF MS strain database is more accurate and large,which can meet the needs of clinical microbiological examination,and is suitable for wider application in clinical microbial identification.
