Surveillance of molecular characteristics of gyrA and parC among fluoroquinolone resistant Streptococcus agalatiae isolates in Suzhou hospitals
10.3969/j.issn.1673-4130.2018.05.002
- VernacularTitle:苏州医院氟喹诺酮耐药无乳链球菌gyrA和parC分子特征调查
- Author:
Xiaoli DAI
1
;
Qianqian SHA
;
Yulin ZHANG
;
Wenxiang LU
;
Qiao ZHONG
Author Information
1. 苏州大学附属第二医院检验科
- Keywords:
Streptococcus agalactiae;
fluoroquinolone;
antibiotic resistance;
gene mutation
- From:
International Journal of Laboratory Medicine
2018;39(5):517-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular characteristic of fluoroquinolone resistant Streptococcus agalactiae (GBS) isolates in Suzhou.Methods Totally 46 fluoroquinolone resistant GBS strains were collected,and then subjected into PCR and two resistance genes were sequenced and their high frequency mutation sites,gyrA and parC were analysed.Results Among these isolates,the most frequent gyrA mutation was gyrA_S81L (TCA→TTA),which displayed 93.4％ (43/46) with substituted at No.81 site of amino acid from Ser to Leu.However,the most frequent parC mutation was mutated at No.79 site of amino acid from Ser to Tyr orPhe,with two mutations parC_S79Y (73.9％,34/46) and parC_S79F(17.4％,8/46).The analysis showed that the major mutation patterns are gyrA_S81L with parC_S79Y (73.9％,34/46),gyrA_S81L with parC(13.0％,6/46).Conclusion The most frequent mutations of fluoroquinolone resistant gene in Suzhou are gyrA and parC and the major mutation patterns are gyrA_S81L/parC_S79Y and gyrA_S81L/parC_S79F.
