Diagnostic value of different detecting technologies in antinuclear antibody in autoimmune disease
10.3969/j.issn.1673-4130.2018.04.022
- VernacularTitle:不同检测技术检测抗核抗体对自身免疫性疾病诊断价值研究
- Author:
Yanhua HUANG
1
;
Weijia WANG
;
Jingjing MA
Author Information
1. 中山大学附属中山医院检验医学中心
- Keywords:
antinuclear antibody;
autoimmune diseases;
indirect immunofluorescence;
enzyme-linked immunosorbent assay;
linear western blot method
- From:
International Journal of Laboratory Medicine
2018;39(4):461-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of different detecting technologies in antinuclear an-tibody(ANA)in autoimmune diseases(AID).Methods A total of 52 cases of patients with systemic lupus er-ythematosus(SLE),97cases of patients with AID but without SLE,92 cases of patients without immune disea-ses were collected in this study.ANA was detected by enzyme-linked immunosorbent assay(ELISA),indirect immunofluorescence(IIFA)method and linear western blot method(LIA).Results Average coincidence rate between ELISA and IIFA method to detect the ANA was 78.8% in the three groups of participants.The re-sult of ANA detected by ELISA method was positively correlated with that of IIFA method(r=0.598,P<0.05).They found that patients with AID and without AID groups both existed the phenomenon of IIFA ANA+LIA ANA -,their fluorescence titers were given priority to with 1:100.Their fluorescence modes were given priority to with granularity in both AID group(67.44%)and without AID group(54.69%),there was no difference between the two groups(P>0.05),but they were significantly higher than that of other flu-orescence modes.At the same time,the study found that patients with IIFA ANA -LIA ANA+were mostly AID patients(73.3%),the ratio was higher than without AID group,among these patients,AID patients were with SSA/Ro60,SSA/Ro52,SSB/La as the main positive autoantibodies,rather than without AID patients with anti-Sm,SSA/Ro52,SSB/La,CenpB as the main positive antibodies.Conclusion It should pay attention to multi-index joint and multi-tests detection when we diagnose autoimmune diseases.
