Establishment and evaluation of different serotypes group B streptococcus method by TaqMan fluorescent probe technique
10.3969/j.issn.1673-4130.2018.04.016
- VernacularTitle:TaqMan荧光探针技术检测不同血清型B群链球菌的方法建立及评价
- Author:
Fan JIANG
1
;
Feiling WANG
;
Xiaoyue LIU
;
Ruijing LU
;
Xiyou ZHOU
;
Jianhua ZOU
Author Information
1. 深圳市宝安区妇幼保健院检验科
- Keywords:
TaqMan fluorescent probe;
real-time fluorescence quantitative polymerase chain reaction;
group B streptococcus
- From:
International Journal of Laboratory Medicine
2018;39(4):438-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a molecular method for the identification of different serotypes of group B streptococcus(GBS)based on TaqMan fluorescence probe technology,and to lay the foundation for the sub-sequent study of multiple fluorescent probe technology to detect different serotypes of GBS.Methods Primers and probes were designed according to the different serotypes of capsular polysaccharide(CPS).CPS se-quences were amplified by real-time fluorescence quantitative polymerase chain reaction.GBS classification methods of different serotypes were established.The results were compared with latex agglutination test and the method was evaluated from the aspects of sensitivity,specificity and detection of clinical isolates.Results The logarithmic concentration of DNA in the same serotype GBS was linearly correlated with the value of Ct. The detection limit of this method is 1 pg/μL,a probe could only detect the corresponding serotype GBS.The results of TaqMan fluorescence probe test of 10 strains were consistent with the results of latex agglutination test.Conclusion TaqMan fluorescence probe technique is a simple,rapid,highly sensitive and specific method for the detection of different GBS serotypes,and it is better than latex agglutination test for the classification of clinical isolates.
