Quantitation of CA199 based on Magnetic Microspheres time resolved fluoroinnnunoassay technology
10.3969/j.issn.1673-4130.2018.04.002
- VernacularTitle:磁性微球时间分辨免疫技术定量检测糖类抗原199的研究
- Author:
Liguo ZHU
1
;
Jun FAN
;
Biao HUANG
;
Hongbo PU
;
Lingfeng PAN
;
Qian SUN
Author Information
1. 江苏省原子医学研究所附属江原医院检验科
- Keywords:
Magnetic Microspheres;
carbohydrate antigen199;
time-resolved fluoroinnnunoassay
- From:
International Journal of Laboratory Medicine
2018;39(4):389-391,395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the time-resolved fluoroimmunoassay(TRFIA)of CA199 based on Mag-netic Microspheres(Nano-TRFIA).Methods Based on a sandwich-type immunoassay format,analytes in sam-ples were captured by magnetic particles coated with anti-CA199 antibody B1 and"sandwiched"by anti-CA199 antibody B7 labeled with europium chelates.A total of 90 serum samples were analysed by this new method. Results The sensitivity was 0.2 U/mL,the intra.and inter.assay CV of the Nano-TRFIA were 4.84% and 8.32% respectively,and the average recovery rate was 97.91%.The cross-reacting rates with alpha fetopro-tein and CA125 were negligible.The labeled B1 with Magnetic Microspheres was at least stable for three months at 4 ℃.Serum samples from patients and healthy blood donors were analyzed,the linear correlation of TRFIA and ECLIA measurements was positive(Y = 0.969 8X+ 4.015 3).As the gold standard of ECLIA, Nano-TRFIA had two false positive.Conclusion The newly developed Nano-TRFIA based on Magnetic Mi-crospheres technique was highly sensitive,stable and specific in the immuno-determination of serum CA199. The results showed that the methods of Nano-TRFIA based on Magnetic Microspheres could be used for the clinic.
