Comparison of rapid detection effects of Mycobacterium tuberculosis in cerebrospinal fluid by RT-LAMP and RT-PCR
10.3969/j.issn.1673-4130.2018.03.019
- VernacularTitle:RT-LAMP与RT-PCR法快速检测脑脊液结核分枝杆菌的效果比较
- Author:
Dandan WU
1
;
Jiwen KANG
;
Dianxing SUN
Author Information
1. 承德医学院
- Keywords:
tuberculous meningitis;
Mycobacterium tuberculosis;
reverse transcription-loop mediated isothermal amplification;
polymerase chain reaction;
16S rRNA
- From:
International Journal of Laboratory Medicine
2018;39(3):322-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of reverse transcription loop-mediated isothermal amplifica-tion(RT-LAMP)method and RT-PCR method for detecting Mycobacterium tuberculosis(MTB)in cerebro-spinal fluid(CSF)to provide a basis for its rapid diagnosis and clinical pharmacodynamic evaluation.Methods Eighty-five cases of CSF sample in the Bethune International Peace Hospital of PLA from December 2015 to April 2017 were selected for conducting the study and divided into the tuberculous meningitis(TBM)group(46 cases),suspected TBM group(25 cases)and control group(16 cases).The 16S rRNA region of MTB was used to design the specific primers.Then RT-LAMP and RT-PCR detection technological systems were estab-lished.Then the detection results by using the these two methods was analyzed.Results The positive detec-tion rates of the TBM group were 97.8% and 75.0% respectively,which of the suspected TBM group were 76.0% and 40.0% respectively,and which of the control group were 0.0% and 12.0% respectively,the posi-tive detection rate of each group in the RT-LAMP method was higher than that in the RT-PCR method,the difference was statistically significant(P<0.01);in the control group,adopting RT-PCR detection found non-specific amplification,while which was not found by adopting RT-LAMP method,indicating that the specifici-ty of RT-LAMP method was stronger than that of RT-PCR;the sensitivity of RT-PCR was 10.0 CFU/mL, which was higher than 1 CFU /mL of RT-LAMP.Conclusion RT-LAMP has the advantages of simpleness,sensitivity,rapidness and detecting viable bacteria,compared with PCR,which has strong specificity,easy op-erating,low cost and short time-consuming,is expected to be a routine detection tool of basic level and field medical institutions and developing countries.
