Study on CMV-DNA detection in blood and urine
10.3969/j.issn.1673-4130.2018.02.016
- VernacularTitle:血液和尿液中巨细胞病毒DNA的检测研究
- Author:
Like ZHANG
1
;
Qianqian CHEN
;
Xuegao YU
;
Bin HUANG
;
Jihong LIU
;
Peisong CHEN
Author Information
1. 惠州市第一人民医院检验科
- Keywords:
cytomegalovirus;
real-time fluorescence quantitative polymerase chain reaction;
nucleic acid extraction;
spin column
- From:
International Journal of Laboratory Medicine
2018;39(2):179-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the detection of cytomegalovirus (CMV)-DNA in different kinds of blood and urine sample .Methods The CMV-DNA loads in 3 different kinds of blood sample from 52 patients and 3 different kinds of urine sample from 85 patients were detected by real-time fluorescence quantitative polymer-ase chain reaction(FQ-PCR) .The differences in CMV-DNA detection rate and virus loads were compared a-mong different kinds of blood and urine samples .Results The CMV-DNA detection rates in serum ,whole blood ,plasma and peripheral blood mononuclear cell (PBMC) from 52 patients were 48 .08％ ,71 .15％ ,57 .69％ and 69 .23％ respectively .The CMV-DNA detection rates of whole blood and PBMC were higher ,the difference was statistically significant (P<0 .05) .The quantitative results of PBMC was higher .The CMV-DNA detec-tion rates of mixed urine ,urine supernatant and urine sediment from 85 patients were 72 .94％ ,62 .35％ and 84 .71％ respectively ,the CMV-DNA detection rate of urine sediment was higher ,while the quantitative re-sults of mixed urine was higher .Conclusion The CMV-DNA detection results of blood and urine have large difference ,therefore using the same kind of sample for conducting detection has an important clinical signifi-cance in the clinical diagnosis ,treatment and monitoring of CM V infection .
