Drug resistance analysis and Staphylococcal cassette chromosome SCCmec genotypes of methicillin resistant Staphylococcus aureus
10.3969/j.issn.1673-4130.2018.02.010
- VernacularTitle:耐甲氧西林金黄色葡萄球菌耐药性分析及SCCmec分型
- Author:
Yuhan ZOU
1
;
Qingzhong LIU
;
Ji ZHANG
;
Lingqin ZHOU
;
Chao CHEN
;
Peiyi YANG
;
Shu JIN
Author Information
1. 上海市普陀区人民医院检验科
- Keywords:
methicillin resistant Staphylococcus aureus;
drug resistance;
SCCmec gene
- From:
International Journal of Laboratory Medicine
2018;39(2):159-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the drug resistance situation and Staphylococcal cassette chromosome mec(SCCmec) genotypes of methicillin resistant Staphylococcus aureus (M RSA ) strains isolated from Shang-hai Putuo District People′s Hospital in order to provide a theoretical basis for predicting the trend of drug re-sistant bacterial strains and clinical treatment and prevention of MRSA .Methods Three hundreds and eighty clinically isolated MRSA strains in this hospital were collected from January 2012 to December 2016 .The in vitro drug susceptibility test was performed by adopting the broth microdilution method .The SCCmec geno-types were examined by adopting the multiplex polymerase chain reaction .Results All strains were sensitive to linezolid and vancomycin ,the sensitivity rate was 100 .0％ ;the resistance rates to rifampicin and cotrimox-azole were lower ,which were 5 .0％ and 7 .6％ respectively ;but the strains were highly resistant to erythromy-cin ,levofloxacin and tetracycline ,with the resistance rate of 100 .0％ ,94 .2％ ,93 .4％ and 90 .0％ .The resist-ance rate to penicillin was 100 .0％ .Among 380 strains of MRSA ,there were 281 strains(73 .9％ ) of SCCmecⅡ ,59 strains (15 .5％ ) of SCCmecⅢand 5 strains (1 .3％ ) of SCCmecⅣa ,other 35 strains(9 .2％ ) of MRSA could not be classified .Conclusion M RSA strains isolated in the Shanghai Putuo District People′s Hospital are mainly the type SCCmecⅡ ,w hich has the multi-drug resistant characteristics ,and the drug resistance spec-trum of different SCCmec genotypes is different .
