Study on upconversion nanoparticles-based rapid fluorescence procalcitonin quantitative detection technology
10.3969/j.issn.1673-4130.2018.01.018
- VernacularTitle:基于上转换纳米荧光快速定量检测PCT技术研究
- Author:
Meng LEI
1
;
Xinqiang XU
;
Tianxing JI
;
Qiang ZHOU
Author Information
1. 广州医科大学附属第二医院检验科
- Keywords:
upconverting fluorescence nanoparticles;
immunochromatographic test strip;
procalcitonin
- From:
International Journal of Laboratory Medicine
2018;39(1):67-69,73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research and develop the immunochromatographic detection technology of upcon-verting nanoparticles(UCNP)-based rapid fluorescence quantitative procalcitonin (PCT ) detection .Methods UCNP (NaYF4 :Yb3+ ,Er3+ ) was prepared by solvothermal method ,which was the compound of rare earth yt-terbium and erbium with four fluorine sodium yttrium .UNCP was conducted the modification and silica (SiO2 ) packing by using the reverse microemulsion method .Then the PCT determination method was estab-lished by using the dry-type immunochromatographic technology .Results The water-soluble UCNP with good dispersibility was successfully prepared by using the reverse microemulsion method .The lowest detection limit for detecting PCT by UCNP-based dry-type immunochromatographic technology was 0 .02 ng/mL ,the linear range was 0 .05 -44 .00 ng/mL ,the intra-batch and inter-batch coefficients variable(CV) were <10％and 13％ respectively .Conclusion UCNP-based immunochromatography technology is a rapidly and sensitively effective method for quantitatively detecting serum PCT .
