Study on HPV infection types distribution in ASC-US ,LSIL and HSIL of uterine cervix
10.3969/j.issn.1673-4130.2018.01.016
- VernacularTitle:宫颈ASC-US、LSIL和HSIL中HPV感染型别分布的研究
- Author:
Yuelan LIANG
1
;
Xue ZHAO
;
Jianxiang GENG
;
Jianyun LAN
;
Zhaoxia YU
;
Sijun XIA
;
Jing MEI
;
Honjing WANG
;
Lin XIA
Author Information
1. 江苏省苏州市吴中人民医院检验科
- Keywords:
uterine cervix;
atypical squamous cells of undetermined significance;
low squamous intra-epithelial lesion;
high squamous intraepithelial lesion;
human papillomavirus
- From:
International Journal of Laboratory Medicine
2018;39(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genotypes distribution and clinical significance of human papillomavir-us(HPV) infection in atypical squamous cells of undetermined significance (ASC-US) ,low squamous intraepi-thelial lesion (LSIL) and high squamous intraepithelial lesion (HSIL) of uterine cervix ,meanwhile to conduct the cervical histopathological diagnostic analysis in the patients with ASC-US、LSIL and HSIL .Methods The gene amplification technique (PCR) combined with gene-chips technology were adopted to conduct the 23 kinds of HPV genotype detection on 236 cases of cervical ASC-US ,36 cases of cervical LSIL and 61 cases of cervical HSIL specimens .All cases of ASC-US ,LSIL and HSIL were performed the cervical biopsy his-topathological diagnosis .And then the subjects related data were analyzed .Results Among 236 cases of cervi-cal ASC-US specimens ,139 cases of HPV infection were detected with the total HPV infection rate 58 .90％(139/236) ,in which the single genotypes infection rate was 38 .14％ (90/236)and the multiple genotypes infec-tion rate was 20 .76％ (49/236);26 cases of HPV infection were detected from 36 cases of cervical LSIL speci-mens with the total HPV infection rate of 72 .22％ (26/36) ,in which the single genotypes infection rate was 52 .78％ (19/36) and the multiple genotypes infection rate was 19 .44％ (7/36);61 cases of HPV infection were detected from 58 cases of cervical HSIL specimens with the total HPV infection rate of 95 .08％ (58/61) , in which the single genotypes infection rate was 68 .85％ (42/61)and the multiple genotypes infection rate was 26 .23％ (16/61) .The total infection rates had statistically significantly differences among the cervical ASC-US group ,LSIL group and HSIL group (P<0 .05) .Conclusion HPV16 ,52 ,58 are the main types in the patients with cervical ASC-US ,LSIL and HSIL .The gene-chip technology can be used in the HPV genotypes detection of cervical cells ,which has an important clinical significance for further distribution management on ASC-US patients and should draw great attention of gynecologist .
