Relationship of miRNA-16 relative expression level and Mayo risk stratification in multiple myeloma
10.3969/j.issn.1673-4130.2018.01.007
- VernacularTitle:多发性骨髓瘤中miRNA-16相对表达量与Mayo危险分层的关系
- Author:
Liangyan SU
1
;
Haiyuan HE
;
Shishi TAO
;
Guomei LIANG
;
Mocan QIU
;
Ke WANG
;
Shunrong LUO
;
Rirong YANG
Author Information
1. 广西医科大学基础医学院
- Keywords:
multiple myeloma;
miRNA-16;
Mayo risk stratification
- From:
International Journal of Laboratory Medicine
2018;39(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the change of miRNA-16(miR-16) level in the patients with multiple my-eloma(MM ) and its relationship with the Mayo risk stratification and prognosis judgment .Methods Each 10 mL of bone marrow samples was collected from 31 cases of MM in the hematology department of the First Af-filiated Hospital of Guangxi Medical University from August 2016 to January 2017 ,and each 5 mL of bone marrow sample was collected from 5 healthy bone marrow donors as the healthy control .The real time fluores-cence quantitative PCR(qRT-PCR) was used to analyze relative the change of miR-16 relative expression level and fluorescence in situ hybridization(iFISH) was used to detect the abnormal gene .Then the relationship be-tween miR-16 relative expression level with Mayo based risk stratification ,and the difference of miR-16 rela-tive expression level between before and after self treatment were statistically analyzed .Results The miR-16 relative expression level in MM patients was increased and positively correlated with the Mayo risk stratifica-tion .Meanwhile in the MM patients with disease progression ,the miR-16 expression was increased . Conclusion miR-16may serve as the indicator for judging the recurrence or progression of MM ,and guides the clinical personalized therapy of MM according to its expression level change .
