Metagenomic analysis of symbiotic microorganism in sailors with long sea voyage
10.3969/j.issn.1673-4130.2018.01.002
- VernacularTitle:长远航海员皮肤共生微生物宏基因组分析
- Author:
Weiwei ZHENG
1
;
Shuyang DAI
;
Yuanyuan QIAO
;
Ze ZHANG
;
Xiaohang ZHAO
Author Information
1. 海军总医院基础医学研究中心
- Keywords:
long sea voyage;
metagenomics;
next generation sequencing;
skin microbias
- From:
International Journal of Laboratory Medicine
2018;39(1):5-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of long sea voyage working environment on the symbiotic microorganisms and their relationship with their hosts .Methods The periumbilical microbial sam-ples from the operating workers of long sea voyage before and after operation were collected .Then 16S rRNA V4 section amplification ,sequencing and whole metagenome shotgun high-throughput sequencing were per-formed .Moreover the bacterial community structure ,kinds and microorganism metabolic function change were analyzed .The peripheral blood was collected from the workers of long sea voyage operation and shore-based operation for conducting the blood routine analysis .Results After 105 d ocean sailing ,the diversity of perium-bilical microbial community in the workers with long sea voyage operation decreased and the relative abun-dance of Firmicutes increased ,w hile w hich of Proteobacteria decreased ;w hich of Staphylococcus increased , while which of Corynebacteria decreased ,the differences were statistically significant (P<0 .05) ,the relative a-bundance of pathogenic bacteria or conditional pathogenic bacteria ,especially Staphylococcus epidermidis and Staphylococcusaureus aureus ,increased significantly .T he functional gene analysis indicated that the expres-sion of periumbilical microbial infection related genes increased after the long sea voyage operation .Compared with shore-based operation workers ,the proportion of workers with peripheral blood lymphocytes abnormal elevation in the long sea voyage group increased significantly ,the difference was statistically significant (P<0 .05) .Conclusion The periumbilical skin symbiotic microorganisms may reflect the health conditions in the workers with long sea voyage operation .
