The expression and significance of N-myc downstream regulated gene-2 in rat kidney after ureteral obstruction
10.3760/cma.j.issn.1673-4408.2018.05.020
- VernacularTitle:N-myc下游调节基因-2在输尿管梗阻大鼠肾脏中的表达及意义
- Author:
Xin QI
1
,
2
;
Yubin WU
Author Information
1. 110004沈阳,中国医科大学附属盛京医院小儿肾内科
2. 117000 本溪市中心医院儿科
- Keywords:
Tubulointerstitial fibrosis;
Ureteral obstruction;
Epithelial mesenchymal transition;
N-myc downstream regulated gene-2
- From:
International Journal of Pediatrics
2018;45(5):405-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of N-myc downstream regulated gene-2 (NDRG2) on renal fibrosis in unilateral ureteral obstruction(UUO) rat model and the mechanism of renal fibrosis.Methods Forty-eight male Wistar rats (120-150g)were randomly divided into two groups:the sham-operation group (n =24)underwent the left ureteral dissection,the UUO group (n =24)underwent the left ureteral ligation.At the 3,7,14,21day after the operation,6 rats from each of the group were sacrificed and the obstructive kidneys were collected.The histopathological changes were observed through HE and Masson staining.E-cadherin and α-SMA were detected by Western blot and immunohistochemistry.NDRG2 were detected by Western blot,immunohistochemistry and real-time PCR.Results There is not diffenrence in the sham group at anytime.Compared with the sham-group,the fibrosis was obvious in UUO group.The protein of E-cadherin decreased(P ＜ 0.05) and α-SMA increased(P ＜0.05).The expression of NDRG2 in UUO group was lower than that in the sham group.The OD of immunohistochemistry was detected by ImagePro Plus 6.0.OD of NDRG2 was the lowest[(14.33 ± 2.45) x 10-3] in 21d group.The western blot showed that the ratio of NDRG2 to β-actin was the lowest(0.03 ± 0.01) in 21d group.The mRNA of NDRG2 decreased obverously in UUO group (P ＜ 0.05).Conclusion NDRG2 decreases in UUO group and NDRG2 might be involved in the pathologic process of renal fibrosis.
