The effect of Clostridium butyricum and Bifidobacterium infantis on sIgA in the intestine of IBD mice
10.3760/cma.j.issn.1673-4408.2018.04.016
- VernacularTitle:酪酸梭菌与婴儿双歧杆菌对炎症性肠病小鼠肠道sIgA的影响
- Author:
Guina WANG
1
;
Zhiqin MAO
Author Information
1. 中国医科大学附属盛京医院小儿消化内科
- Keywords:
sIgA;
Inflammatory bowel disease;
Clostridium butyricum;
Bifidobacterium infantis;
Mice
- From:
International Journal of Pediatrics
2018;45(4):311-314,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the inflammatory bowel disease model of mice by Trinitro-benzenesulfonic acid(TNBS).To investigate the correlation between the IBD and SIgA level and the effect of Clostridium butyricum,Bifidobacterium infantis single or combined on sIgA expression.Methods BABL/c mice were randomly divided into:WT group,TNBS group,CB group,BB group and CB + BB group.After 2 weeks,the general situation and weight change of mice was evaluated.Then the mice were sacrificed to collect colon tissue.The inflammation of each group was observed by HE staining.The expression of sIgA were respectively located and measured by immunohistochemistry and Western blotting.Results TNBS group showed more pathological changes in the colon than the WT group,CB group,BB group and CB + BB group;the expressions of sIgA in colon were mainly located in intestinal lumen and lamina propria of intestinal villus;the level of sIgA in colon and tissue decreased in the TNBS group compared with WT group(P ＜ 0.01;P ＜ 0.01);the level of sIgA in colon and tissue increased ín the CB group and BB group compared with TN-BS group(P ＜0.01;P ＜0.01;P ＜0.01;P ＜ 0.01).There is no significant difference between CB group and BB group(P ＞ 0.05).The level of sIgA in colon and tissue increased in the CB +BB group compared with CB group or BB group(P ＜0.01;P ＜0.01).Conclusion The change of sIgA content is related to the occurrence of IBD.CB and BB can both promote sIgA production in IBD mice and reduce the inflammatory reaction.The combination of the two probiotics shows stronger effect than single one.
