The effects of hyperoxia on alveolar epithelial cells of neonatal rats with bronchopulmonary dysplasia
10.3760/cma.j.issn.1673-4408.2017.12.019
- VernacularTitle:高氧对支气管肺发育不良新生鼠肺泡上皮细胞的影响
- Author:
Dongmei YUE
1
;
Yajie TONG
;
Xindong XUE
Author Information
1. 中国医科大学附属盛京医院新生儿科
- Keywords:
Hyperoxia;
Alveolar epithelial cells;
Bronchopulmonary dysplasia;
Aquaporin
- From:
International Journal of Pediatrics
2017;44(12):891-895
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of alveolar epithelial cells (AEC) on bronchopulmonary dysplasis(BPD) induced by hyperoxia in newborn rats.Methods The model of BPD induced by hyperoxia in neonatal rats was established.HE staining was used to observe the alveolar septum and alveoli development.The expression of the specific surfactant protein C (SPC) on type Ⅱ alveolar epithelial cells (AEC Ⅱ) and the expression of specific aquaporin 5 (aquaporin5 AQP5) on type Ⅰ alveolar epithelial cells (type Ⅰ alveolar epithelialcells,AECⅠ) were measured by immunohistochemistry.Results There was no significant difference of AQP5 between experimental groups and control group at 1 d,but significantly lower expression of AQP5 could be seen in experimental group1 on day3 (P ＜ 0.05).And then,the AQP5 level of lung tissue of newborn rats at 5,7,14d after experiment in groupl was significantly lower than that of in air control group (P ＜ 0.05).The expression of SPC protein had no significant difference between experimental groups and control group at 1 d,but the SPC level of lung tissue of newborn rats at 3,7,14d after experiment in group1 was significant lower than that of in air control group (P ＜ 0.05).Conclusion Hyperoxia exposure leads to the expression of SP-C and AQP5 decreased,which may be key points for the development of BPD.AECⅡ may play an important role in the repairation of alveolar epithelial cells in neonatal rats with BPD induced by hyperoxia.
