Establishment of DNA Genetic Marker Identification System for Plant Evidence
10.3969/j.issn.1004-5619.2018.02.006
- VernacularTitle:植物类物证DNA遗传标记鉴定系统的建立
- Author:
Hui LI
1
;
Pan XIA
;
Chuang WANG
;
Yuan WANG
;
Xue-Ying ZHAO
;
Ke MA
;
Wei DAI
;
Yu CAO
;
Huai-Gu ZHOU
;
Wen-Bin LIU
Author Information
1. 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室 上海市刑事科学技术研究院
- Keywords:
forensic genetic;
DNA,plant;
genetic markers;
species identification
- From:
Journal of Forensic Medicine
2018;34(2):138-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a species identification system based on DNA genetic markers for plant evidence. Methods Two hundred common plants in Shanghai were collected and identified by mor-phological characteristics. The primers of gene segments rbcL, matK, and ITS were designed and amplified. The PCR amplicon was detected by agarose gel electrophoresis. After the sequencing, the universality and the identification capacity of the three markers were evaluated. Results The success rate of amplifi-cation was in order of rbcL (99.5％) >matK (92.5％) > ITS (86.0％). The identification capacity of the combination of rbcL and matK was better than that of rbcL or matK, by which most plant species could be identified to the genus or higher. ITS was not suitable to be a unique marker because of its unstable result, but it still could be a powerful supplement. The identification capacity of the combination of rbcL, matK and ITS was higher than that of rbcL and matK, by which most plant species could be identified to the genus or lower. Conclusion The identification system with the combination of rbcL, matK and ITS as markers has excellent universality for plant evidence, which can distinguish most plant species to the genus or lower.
