Establishment of 43-plex SNP Typing System and Its Forensic Application
10.3969/j.issn.1004-5619.2018.02.004
- VernacularTitle:43个SNP遗传标记复合检验体系的建立及其法医学应用
- Author:
Ya-Nan LI
1
;
Min LI
;
Lei JIANG
;
Xiao-Hui LUAN
;
Na LIANG
;
Qian-Nan XU
;
Jia-Shuo ZHANG
;
Ming-Chi TANG
;
Ying-Nan BIAN
;
Li-Qin CHEN
Author Information
1. 内蒙古医科大学基础医学院
- Keywords:
forensic genetics;
polymorphism,genetic;
matrix-assisted laser desorption-ionization;
mass spectrometry;
single nucleotide polymorphism;
Han nationality;
Eastern China
- From:
Journal of Forensic Medicine
2018;34(2):126-131,137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of 43-plex SNP typing system in forensic science. Methods The typing of 43 SNP loci in 123 unrelated Han individuals from East China was detected by MALDI-TOF-MS. The application value of 43-plex SNP typing system was assessed according to the foren-sic parameters of population genetics. Results All the 43 SNP loci of 123 individuals showed no signifi-cant departure from Hardy-Weinberg equilibrium (P>0.05). Excepted rs1355366, rs2270529, rs10776839 and rs938283, there were 39 SNP loci had minor allele frequencies (MAF), which were greater than 0.25. Among the 25 loci MAFs, 24 ranged from 0.4 to 0.5, while 3 were close to 0.4. The DP, CDP, PIC, Ho, PEtrio and PEduo of the 43 SNP loci were 0.2901-0.6544, 1-9.8×10-11, 0.1708-0.5000, 0.1557-0.5935, 0.0854-0.2500 and 0.0146-0.1250, respectively. The CPEtrio and CPEduo were 0.999986 and 0.9924361, respectively. Conclusion The 43-plex SNP typing system in present study shows a high polymorphism, which can be an effective supplement and verification for traditional STR genetic markers. It also can be used with other commercial kits for the forensic paternity testing and individual identification.
