Forensic Application of SiFaSTRTM 23plex DNA ID System in Han Population of Eastern China
10.3969/j.issn.1004-5619.2018.02.003
- VernacularTitle:SiFaSTRTM 23plex DNA身份鉴定系统在华东汉族人群中的法医学应用
- Author:
Yun BAO
1
;
Xiang SHENG
;
Jia-Shuo ZHANG
;
Min LI
;
Ya-Nan LI
;
Qian-Nan XU
;
Cheng-Tao LI
;
Li-Qin CHEN
Author Information
1. 内蒙古医科大学基础医学院
- Keywords:
forensic genetics;
polymorphism,genetic;
DNA mutational analysis;
short tandem repeat;
Han nationality;
Eastern China
- From:
Journal of Forensic Medicine
2018;34(2):120-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic polymorphism of 21 autosomal STR loci and DY S391 locus of SiFaSTRTM 23plex DNA ID system in Han population of eastern China and to evaluate its ap-plication value in forensic science. Methods Typing test of 2000 unrelated individuals was performed using SiFaSTRTM 23plex DNA ID system. The population genetic parameters of STR loci were statistically analysed. A total of 3198 parentage confirmed cases were detected with that system and the mutation conditions were observed in 21 autosomal STR loci. Results All the 21 autosomal STR loci showed no significant departure from Hardy-Weinberg equilibrium (P>0.05). The Ho ranged from 0.6175 to 0.9270. The DP ranged from 0.7964 to 0.9869, as well as the PIC distributed from 0.5611 to 0.9123. The CDP was 0.999999999999999. The CPEduo was 0.999997431701961, while CPEtrio was 0.999999999654865. Five alleles were detected in DY S391 locus, with the allele frequency from 0.0040 to 0.7290, and GD was 0.4189. Except D13S317 and D10S1248, seventy-six mutation events were observed at the rest nineteen autosomal STR loci. Among them, seventy-five (98.68％) were one step mutation, and only one (1.32％) was three steps mutation. The mutation rate ranged from 0.2465×10-3 to 2.7114×10-3, and the averaged mutation rate was 0.8921×10-3 (95％ CI: 0.70×10-3-1.10×10-3). In 33 trio mutation cases, the proportion of the paternal mutation and the maternal mutation was 2.09:1. Conclusion The involved STRs are highly polymorphic in Eastern Han population with acceptable mutation rates by the SiFaSTRTM 23plex DNA ID system, which is suitable for paternity testing and individual identification.
