Expressions of IL-1α, IL-1β and IL-13 mRNA in Lung Tissue and Serum of Rats Drown in Fresh Water
10.3969/j.issn.1004-5619.2018.02.001
- VernacularTitle:淡水溺死大鼠肺组织与血清中IL-1α、IL-1β和IL-13 mRNA表达
- Author:
Mei-Ling JIANG
1
;
Zhi-Jie LIU
;
Jing PAN
;
Jian LU
;
Xin-Hua LIANG
Author Information
1. 吉林鸣正司法鉴定中心
- Keywords:
forensic pathology;
drowning;
fresh water;
interleukin-1 alpha;
interleukin-1 beta;
interleukin-13;
rats
- From:
Journal of Forensic Medicine
2018;34(2):111-113,119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the changes of (interleukin, IL) -1α, IL-1β and IL-13 mRNA in lung tissue and serum of drown rats, and to explore the potential value for the diagnosis of drowning in forensic practice. Methods Eighteen SD rats were randomly divided into drowning group, blank control group and myocardial infarction group (as control group). The serum of right ventricular, the inferior lobe of right lung and the myocardium were taken from the rats in different groups. The expressions of IL-1α, IL-1β and IL-13 mRNA in the lung tissue and the serum of right ventricular were detected by TaqMan probe method. Results The expression differences of IL-1α, IL-1β and IL-13 mRNA in lung tissue between drowning group and blank control group, myocardial infarction group were not statistically significant (P>0.05). The expression of IL-1β and IL-13 mRNA in serum of right ventricular increased (P<0.05). The expression differences of IL-1α, IL-1β and IL-13 mRNA in serum between blank con-trol group and myocardial infarction group were not statistically significant (P>0.05). Conclusion The changes of cytokines IL-1β and IL-13 mRNA in the serum of right ventricular of drown rats are statis-tical significance, which are highly correlated with drowning.
