Determination of 1-methylhydantoin Concentration in Blood by GC-MS Method and Its Application in Forensic Medicine
10.3969/j.issn.1004-5619.2017.06.011
- VernacularTitle:GC-MS法测定血中1-甲基海因含量及其法医学应用
- Author:
Li-Na GAO
1
;
Hui-Ya YUAN
;
En-Yu XU
;
Jun-Ting LIU
Author Information
1. 中国医科大学法医学院
- Keywords:
forensic toxicology;
gas chromatography-mass spectrometry;
blood;
1-methylhydantoin;
metabo-lite
- From:Journal of Forensic Medicine
2017;33(6):619-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a gas chromatographic-mass spectrometric (GC-MS) analysis method for quantifying 1-methylhydantoin concentration in whole blood. To provide technical support to forensic identification related cases of 1-methylhydantoin. Methods As an internal standard, 500 ng SKF525A was added to 0.5 mL blood sample, and then 2 mL 0.01 mol/L dilute hydrochloric acid and 0.5 g ammonium carbonate were added in order to buffer the pH value to 9, and following 2 mL ethyl acetate. The organic solvent layer was obtained after centrifuge and then analysed by GC-MS after drying. Results Good linear relationship of 1-methylhydantoin in blood was obtained in the range of 0.5-50 ng/mL. The equation of linear regression was y=0.01551 x+0.00726(R2=0.9997) with 0.1 ng/mL detection limit, and the recovery was 93.02％-108.12％. The intra-day and inter-day precision were less than 6.07％ and 13.37％, respec-tively. Conclusion The results gotten by this method is accurate and reproducible, which can be used for the determination of 1-methylhydantoin concentration in blood samples.
