Determination of Hair Shafts by InnoTyper? 21 Kit
10.3969/j.issn.1004-5619.2017.06.010
- VernacularTitle:应用InnoTyper?21试剂盒进行毛干检验
- Author:
Fu LI
1
;
Min ZHANG
;
Ying-Xi WANG
;
Jing-Jing SHUI
;
Meng YAN
;
Xiao-Pan JIN
;
Xiao-Jun ZHU
Author Information
1. 北京市东城区公安司法鉴定中心
- Keywords:
forensic genetics;
insertion/deletion;
hair shaft;
personal identification;
InnoTyper? 21 kit
- From:Journal of Forensic Medicine
2017;33(6):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application value of InnoTyper? 21 kit in forensic practice. Methods Samples of hair shafts and saliva were collected from 8 unrelated individuals. Template DNA was ex-tracted by AutoMate ExpressTM forensic DNA automatic extraction system. DNA was amplified by Inno-Typer? 21 kit and AmpFeSTRTM IdentifilerTM Plus kit, respectively, and then the results were compared. Results After the amplification by InnoTyper ? 21 kit, complete specific genotyping could be detected from the saliva samples, and the peak value of genotyping profiles of hair shafts without sheath cells was 57-1219 RFU. Allelic gene deletion could be found sometimes. When amplified by AmpFeSTRTM IdentifilerTM Plus kit, complete specific genotyping could be detected from the saliva samples , and the specific fragment was not detected in hair shafts without sheath cells. Conclusion The InnoTyper? 21 kit has certain application value in the cases of hair shafts without sheath cells.
