Genetic Polymorphism of 30 InDel Loci in Han Population from Jiangsu Province
10.3969/j.issn.1004-5619.2017.06.009
- VernacularTitle:江苏汉族人群30个插入/缺失位点的遗传多态性
- Author:
Meng PAN
1
;
Xiao-Bin JU
;
Yan-Ting LIU
;
He CUI
;
Min GU
;
Hui-Ying ZHOU
Author Information
1. 南京医科大学第一附属医院 江苏省人民医院司法鉴定所
- Keywords:
forensic genetics;
polymorphism;
genetic;
insertion/deletion;
Investigator ? DIPplex kit;
Han nationality;
Jiangsu
- From:
Journal of Forensic Medicine
2017;33(6):611-614,618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic information of 30 insertion/deletion (InDel) loci in Han population from Jiangsu Province, and to explore the application values of Investigator? DIPplex kit for guiding the forensic analysis in Han population from Jiangsu Province. Methods The autosomal InDel loci of 305 unrelated healthy Han individuals from Jiangsu Province were genotyped and analysed by In-vestigator? DIPplex kit, and the allelic frequencies and forensic parameters of 30 InDel loci were statis-tically analysed. Results The distribution of 30 InDel loci in Han population from Jiangsu Province con-formed to Hardy-Weinberg equilibrium. The minor allele frequencies of 21 InDel loci were above 0.3. The polymorphism information content ranged from 0.089 to 0.375, while the discrimination power dis-tributed from 0.093 to 0.500. The paternity exclusion in duo cases and trio cases were 0.047-0.250 and 0.046-0.219, respectively. The linkage disequilibrium analysis of 30 InDel loci showed that all loci were independent from each other. The combined discrimination power was 1-7.369 ×10-8, whereas the com-bined mean exclusion chance in duo cases was 0.998933978, in trio cases was 0.997806392. The Fst values were all less than 0.06 except HLD118 and other four loci, which showed small differences be-tween groups. Conclusion The InDel loci of Investigator ? DIPplex kit can be used as complementary genetic markers for the cases associated with forensic genetics.
