Identification of Methamphetamine Abuse and Selegiline Use: Chiral Analysis of Methamphetamine and Amphetamine in Urine
10.3969/j.issn.1004-5619.2017.06.006
- VernacularTitle:甲基苯丙胺滥用与司来吉兰服用的区分:尿液中甲基苯丙胺和苯丙胺的手性分析
- Author:
Ping XIANG
1
;
Jun BU
;
Zheng QIAO
;
Xian-Yi ZHUO
;
He-Jian WU
;
Min SHEN
Author Information
1. 司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台
- Keywords:
forensic toxicology;
methamphetamine;
amphetamine;
selegiline;
chiral analysis;
LC-MS/MS;
urine
- From:Journal of Forensic Medicine
2017;33(6):599-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the content variation of selegiline and its metabolites in urine, and based on actual cases, to explore the feasibility for the identification of methamphetamine abuse and selegiline use by chiral analysis. Methods The urine samples were tested by chiral separation and LC-MS/MS method using CHIROBIOTICTM V2 chiral liquid chromatography column. The chiral analysis of metham-phetamine and amphetamine were performed on the urine samples from volunteers of selegiline use and drug addicts whom suspected taking selegiline. Results After 5 mg oral administration, the positive test time of selegiline in urine was less than 7 h. The mass concentrations of R(-)-methamphetamine and R(-)-amphetamine in urine peaked at 7 h which were 0.86μg/mL and 0.18μg/mL and couldn't be de-tected after 80 h and 168 h, respectively. The sources of methamphetamine and amphetamine in the urine from the drug addicts whom suspected taking selegiline were analysed successfully by present method. Conclusion The chiral analysis of methamphetamine and amphetamine, and the determination of selegi-line's metabolites can be used to distinguish methamphetamine abuse from selegiline use.
