Microfluidic Chip for Methamphetamine Detection Based on Time-resolved Fluoroimmunoassay
10.11895/j.issn.0253-3820.181216
- VernacularTitle:基于时间分辨免疫分析的冰毒检测微流控芯片
- Author:
Jing-Jing SUN
1
;
Qi SONG
;
Ying MU
Author Information
1. 浙江大学智能系统与控制研究所
- Keywords:
Time-resolved fluoroimmunoassay;
Methamphetamine;
Competitive immunoassay;
Microfluidic chip
- From:
Chinese Journal of Analytical Chemistry
2018;46(7):1116-1121
- CountryChina
- Language:Chinese
-
Abstract:
A novel polydimethylsiloxane ( PDMS)-poly-L-lysine ( PLL) microfluidic chip, comprised of 24 reaction channels with 2. 5 μL volume of each reaction channel only, was proposed for quantitative analysis of methamphetamine ( MET) based on time-resolved immunoassay techniques. The chip utilized the adsorption characteristics of PDMS and PLL towards proteins to immobilize MET complete antigens ( MET-BSA) on the surface of reaction channel. Then the competition reaction could happen between MET-Ag in the sample solution and MET-BSA on the inner surfaces of the reaction channels with MET-Ab in the reagent. The surface of latex microspheres was labeled by lanthanide, which could emit red fluorescence under the exposure of ultraviolet ( UV ) . Based on the principle of competitive immunoassay, the more MET-Ag, the less the fluorescence intensity in the reaction channel. The detection results of this chip were acquired using UV-irradiation fluorescence imaging method. With this method, 24 samples could be detected and analyzed simultaneously on a chip by just taking the fluorescence image of the chip. The method allows the detection of MET antigens ranging from 100 ng/mL to 3000 ng/mL, with less sample consumption and high-throughput. This chip is suitable for the police preliminary screening work and has a good application prospects.
