Label-free Fluorescent DNA Detection Strategy Based on Hybridization Chain Reaction and Vacant Site-Binding Molecule
10.11895/j.issn.0253-3820.181142
- VernacularTitle:基于DNA杂交链式反应和杂交空位的无标记荧光检测DNA研究
- Author:
Jie XU
1
;
Bin LIN
;
Ya WANG
;
Zhi-Ai XU
;
Gui-Ying CHENG
;
Wen ZHANG
Author Information
1. 华东师范大学化学与分子工程学院
- Keywords:
Hybridization chain reaction;
Vacant site;
Fluorescence;
Signal amplification;
Label-free
- From:
Chinese Journal of Analytical Chemistry
2018;46(7):1095-1101
- CountryChina
- Language:Chinese
-
Abstract:
A label-free and enzyme-free fluorescent DNA sensor based on hybridization chain reaction ( HCR) and vacant site-binding molecule was developed. By rationally incorporating a cytosine in hairpin DNAs for HCR and utilizing vacant site-binding fluorophore ATMND, sensitive DNA detection was achieved, resulting from the large amount of vacant sites presented on the long DNA nanostructures upon HCR. Gel electrophoresis and atomic force microscopy were performed to characterize the hybridization chain reaction. Under the optimized conditions, the method could sensitively detect the target DNA with the low detection limit of 2. 0 nmol/L. The method shows great potential in signal amplification for DNA detection with advantages such as simple, label-free and low-cost.
