Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
10.11895/j.issn.0253-3820.171285
- VernacularTitle:功能化磁性纳米粒子联合质谱技术用于金属硫蛋白的富集及鉴定研究
- Author:
Xin-Yu HE
1
;
Bing WANG
;
Yang-Yang ZHOU
;
Xiao-Jun BIAN
;
Juan YAN
Author Information
1. 上海海洋大学食品学院
- Keywords:
Metallothionein;
Low-abundant;
Magnetic nanomaterials;
Gold nanoparticles;
Core-shell structure;
Mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2018;46(7):1069-1076
- CountryChina
- Language:Chinese
-
Abstract:
Metallothionein ( MT) is a low-molecular-weight protein with high inducibility and binding ability with metal ions. Therefore, MT is often regarded as an important biomarker for assessment of heavy metal pollution in water environment. But the traditional process of its enrichment and identification is time-consuming and complicated. Herein, we prepared a core-shell nanoparticle, gold-coated iron oxide nanoparticles ( Fe3O4@Au NPs) . The nanoparticle possessed the advantages such as fast response to magnetic fields and optical properties attributing to Fe3O4and Au nanoparticles separately. Fe3O4@Au nanoparticles were used to enrich MT simply through Au-S interaction, and the purified proteins were determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry ( MALDI-TOF/MS) . The results in this work showed that the Fe3O4@Au nanoparticles could directly enrich MT from complex solutions and the detection limit could be down to 10 fg/mL.
