Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications
10.11895/j.issn.0253-3820.181110
- VernacularTitle:二次离子质谱-激光扫描共聚焦显微镜联用对单细胞显微成像-原理及应用
- Author:
Chang-Fang SHAO
1
;
Yao ZHAO
;
Kui WU
;
Fei-Fei JIA
;
Qun LUO
;
Zhe LIU
;
Fu-Yi WANG
Author Information
1. 曲阜师范大学抗癌药物开发与诊疗应用研究所
- Keywords:
Secondary ion mass spectrometry;
Laser scanning confocal microscopy;
Correlated secondary ion mass spectrometry and laser scanning confocal microscopy imaging;
Single cell imaging;
Cell biology;
Review
- From:
Chinese Journal of Analytical Chemistry
2018;46(7):1005-1016
- CountryChina
- Language:Chinese
-
Abstract:
Secondary ion mass spectrometry ( SIMS) as a powerful surface analysis technique has been widely applied in semiconductor industry and geology research. Recently, with the development of instrumental technology, SIMS is attracting more and more attention in life sciences. SIMS can provide surface MS spectra, 2D/3D chemical images and depth profiling of substances simultaneously. The minimal lateral resolution of 2D SIMS imaging is 80 to 100 nm, and the longitudinal resolution of 3D SIMS imaging is about 1-5 nm. However, due to lack of specific ions to render the structures of organelles, SIMS imaging for single cells still has great challenges. Optical microscopy, in particular laser scanning confocal microscopy ( LSCM) , has been emerged to be an indispensable technique for single cell imaging and can obtain high spatial 2D/3D imaging to visualize the structures of organelles. Thus, the combinational use of SIMS and LSCM, which takes advantages of SIMS for molecular imaging and LSCM for morphological imaging, has greatly extended the application of SIMS imaging and ensured its accuracy at single cells level, providing novel insights into better understanding of the biological events inside cells. In this review, we focus on the development and application of SIMS imaging and the correlated SIMS and LSCM imaging in the research of cell biology and drug discovery. We anticipate that the combinational use of SIMS and LSCM imaging has promising future in biomedicine and life sciences.
