A Fast Analytical Approach for Determination of 16 Kinds of Mycotoxins in Vegetable Oils Using Stable Isotope Dilution and Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

VernacularTitle:稳定同位素稀释-超高效液相色谱-串联质谱法快速测定植物油中16种真菌毒素
Author: Yu WU ¹ ; Jin YE ; Bing ZHANG ; Song-Shan WANG ; Li LI ; Rui LI ; Sen LI ; Hua CUI ; Gang XIE ; Song-Xue WANG ; Xiao-Ming LI
Author Information

1. 国家粮食局科学研究院
Keywords: Stable isotope dilution technique; Ultra high performance liquid chromatography-tandem mass spectrometry; Vegetable oils; Mycotoxins
From: Chinese Journal of Analytical Chemistry 2018;46(6):975-984
CountryChina
Language:Chinese
Abstract: A fast, simple and cost-effective UPLC-MS / MS method was established for determination of 16 kinds of mycotoxins in vegetable oils with stable isotope dilution technique. Samples were extracted by acetonitrile-water-acetic acid 84: 15: 1(V/ V) and then diluted using water without any further clean-up steps. The mycotoxins were fully separated on a pentafluorophenyl column. Matrix effects were efficiently compensated by the [ 13 C]-labelled internal standards. The mean recoveries at three different concentration levels ranged from 74. 2％ to 105. 6％ , with RSD varied from 0. 3％ to 13. 9％ . Finally, the method was applied to analyze several kinds of vegetable oil samples. The method was simple, rapid, high sensitive and suitable for the determination of mycotoxins in vegetable oils.