Detection of Arginine by AuNPs / CQDs Nanoprobes Based on Fluorescence Resonance Energy Transfer Effect
10.11895/j.issn.0253-3820.181096
- VernacularTitle:基于荧光共振能量转移的 金纳米粒子/碳量子点荧光纳米探针检测精氨酸
- Author:
Xiao-Bo ZOU
1
;
Yong-Qiang SHI
;
Yue ZHENG
;
Ji-Yong SHI
;
Xue-Tao HU
;
Cai-Ping JIANG
;
Xiao-Wei HUANG
;
Yi-Wei XU
Author Information
1. 江苏大学食品与生物工程学院
- Keywords:
Arginine;
fluorescent probe;
Fluorescence resonance energy transfer;
Carbon quantum dots;
Gold nanoparticles
- From:
Chinese Journal of Analytical Chemistry
2018;46(6):960-968
- CountryChina
- Language:Chinese
-
Abstract:
A novel method for rapid detection of arginine based on fluorescence resonance energy transfer effect (FRET) between carbon quantum dots ( CQDs) and gold nanoparticles ( AuNPs) was developed. Firstly, the CQDs with excellent fluorescence properties were synthesized by one-step microwave assisted method. The AuNPs/ CQDs composites were characterized and their quenching mechanism was analyzed. Then the amount of AuNPs/ CQDs, the pH value and the reaction time were optimal. Under the optimum conditions, the fluorescence system was used to detect the content of arginine, showing a good linear relationship ( R2 = 0. 993 ) between fluorescence intensity and concentration of arginine in the range of 0. 1-10. 0 μmol/ L, and the detection limit was 5. 8 nmol/ L. Finally, the content of arginine in grape juice was determined by this method with recoveries of 105. 4％ -110. 8％ , which indicated that the proposed FRET system had the potential for practical detection of arginine in fruit juice.
