Fluorescence Carbon Dot-based Imprinted Polymer for Highly Selective Detection of Bovine Hemoglobin
10.11895/j.issn.0253-3820.171417
- VernacularTitle:碳点基荧光印迹聚合物选择性检测牛血红蛋白
- Author:
Piao-Piao LYU
1
;
Dan-Dan XIE
;
Zhao-Hui ZHANG
Author Information
1. 吉首大学化学化工学院
- Keywords:
Molecular imprinting;
Bovine hemoglobin;
Fluorescent carbon dots
- From:
Chinese Journal of Analytical Chemistry
2018;46(6):917-924
- CountryChina
- Language:Chinese
-
Abstract:
A novel fluorescent imprinted polymer (CDs@ MIP) with selective recognition of hemoglobin was prepared by the sol-gel method using fluorescent carbon dots as the carrier material, 3-aminopropyltrieth-oxysilane as the functional monomer, tetraethoxysilane as the crosslinking agent and bovine hemoglobin as template molecule. The results of IR and scanning electron microscopy showed that the molecularly imprinted polymer was coated on the surface of fluorescent carbon dots. The CDs@ MIP showed selective recognition properties for bovine hemoglobin with an imprinting factor of 4. 60. Also the adsorption ability and specific recognition performance of CDs@ MIP were investigated, and it was found that the CDs@ MIP had high selectivity toward bovine hemoglobin, and the selection factors for ovalbumin, bovine serum albumin and human serum albumin were 4. 38, 4. 73 and 3. 66, respectively. Under the optimal conditions, the linear range of CDs@ MIP for bovine hemoglobin was 0. 1-10. 0 μmol/ L and the detection limit was 23. 0 nmol/ L. The CDs@ MIP was successfully used for the determination of bovine hemoglobin in bovine blood samples with recoveries of 99. 0％ -102. 5％ .
