Real-time Event Recognition and Analysis System for Nanopore Study
10.11895/j.issn.0253-3820.171476
- VernacularTitle:纳米孔道单分子电化学信号在线识别与分析研究
- Author:
Hui-Feng WANG
1
;
Fei HUANG
;
Zhen GU
;
Zheng-Li HU
;
Yi-Lun YING
;
Bing-Yong YAN
;
Yi-Tao LONG
Author Information
1. 华东理工大学信息科学与工程学院
- Keywords:
Nanopore;
Data acquisition and storage;
Real-time data analysis and processing;
Data buffering;
Adaptive threshold algorithm
- From:
Chinese Journal of Analytical Chemistry
2018;46(6):843-850
- CountryChina
- Language:Chinese
-
Abstract:
To achieve fast and accurate analysis of weak current signal of nanopore-based single molecule detection, we designed a real-time adaptive threshold data processing algorithm with data buffering technique and finite impulse response filtering. The system, which is designed based on the data processing algorithm, could realize real-time recognition and analysis of nanopore events during the data recording process. In order to verify the performance of the system, the ideal signals with different noise level (20-100 pA) and recording bandwidth (3 - 100 kHz) was generated. The results showed that the system was stable to analyze the generated signals even at high noise. In addition, the system was also suitable for the data recording conditions of low bandwidth and high sampling rate (250 kHz). The proposed nanopore data processing system was further applied in the Aerolysin nanopore experiment for the detection of poly(dA) 4 molecules. The results showed that the data processing system could be applied in real nanopore recording system with high accuracy and speed.
