Simultaneous Determination of Ten Banned Substances in Pig Urine by Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.170127
- VernacularTitle:液相色谱-串联质谱同时监测猪尿中10种违禁药物
- Author:
Jing-Meng XIE
1
;
Chen-Ying MENG
;
Er-Fen LI
;
Ying-Xia ZHANG
;
Xu-Qin SONG
;
Mei-Yu ZHANG
;
Li-Min HE
Author Information
1. 国家兽药残留基准实验室(华南农业大学)
- Keywords:
Banned substances;
Lyophilization method;
Liquid chromatography-tandem mass spectrometry;
Swine urine
- From:
Chinese Journal of Analytical Chemistry
2018;46(5):750-756
- CountryChina
- Language:Chinese
-
Abstract:
A simple, sensitive and reliable method was developed for simultaneous determination of ten banned drugs residues including zeranols(ZALs),chloroamphenicol,pentachlorophenol,etc. in swine urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The urine samples were pretreated using lyophilization and QuEChERS procedures, respectively. Acetonitrile and ammonium acetate (5 mmol/L) were chosen as mobile phases. Target compounds were separated well in ZorbaxSB-C18by following the optimized gradient elution program and determined by LC-MS/MS in negative electrospray ionization mode. The linearity of the matrix-matched standard curve of ten analytes in two methods was good in the range of the experimental concentration with correlation coefficients more than 0.99. The recoveries of ten drugs were in the range of 80.7%-107.7% and 73.5%-103.3% at the spiked levels of 5,10 and 20 μg/L by lyophilization and QuEChERS methods,respectively. The coefficients of variation were less than 15%. The limits of detection (LOD) and the limits of quantification (LOQ) from lyophilization and QuEChERS method were 0.1 to 2.0 μg/L and 0.2 to 5.0 μg/L,respectively.
