Determination of Pyraclostrobin and Its Metabolite Residues in Pollens and Honeys of Litchi By Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.171249
- VernacularTitle:超高效液相色谱-串联质谱法测定荔枝花粉花蜜中吡唑醚菌酯及其代谢物
- Author:
Si-Wei WANG
1
;
Yan-Ping LIU
;
Hai-Bin SUN
Author Information
1. 广东省农业科学院 植物保护研究所/广东省植物保护新技术重点实验室
- Keywords:
Ultra performance liquid chromatography-tandem mass spectrometry;
Pyraclostrobin;
Metabolite;
Pollen;
Honey
- From:
Chinese Journal of Analytical Chemistry
2018;46(5):704-709
- CountryChina
- Language:Chinese
-
Abstract:
An effective method was developed for determination of pyraclostrobin and BF 500-3 residues in pollen and honey of litchi by QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry. The samples were extracted by acetonitrile and cleaned by primary secondary amine(PSA) and C18. In the positive ion mode and multi reaction monitoring mode,the analytes were quantified by the matrix matching standard solutions, and the pretreatment and mass spectrometry conditions were evaluated. The matrix matched standard solutions of pyraclostrobin and its metabolite BF 500-3 showed good linearities in the concentration range of 1-100 μg/L, with the correlation coefficients (R2) of 0.991-0.999. The average recoveries were 87.0%-97.8% with relative standard deviations of 1.3%-3.7%. The limit of detection (LOD) and the limit of quantification(LOQ) were 0.08-0.20 μg/kg and 0.20-0.50 μg/kg,respectively. The method was easy, quick and highly sensitive, and was suitable for quick determination of pyraclotrobin and its metabolites in pollen and honey of litchi.
