Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion
10.11895/j.issn.0253-3820.171421
- VernacularTitle:基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究
- Author:
Yang YU
1
;
Jia-Ze YAN
;
Yan JIN
Author Information
1. 中国科学院大连化学物理研究所
- Keywords:
Deer antler;
Simulated gastrointestinal digestion;
Reversed phase liquid chromatography-tandem mass spectrometry;
Peptide profiling;
Biological activities
- From:
Chinese Journal of Analytical Chemistry
2018;46(4):601-608
- CountryChina
- Language:Chinese
-
Abstract:
Reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) was utilized to investigate peptide profiling and bioactivities of antler aqueous extract(AAE),digested antler aqueous extract (AED) and powder(PD). A total of 23,417 and 389 peptides,as well as 15,146 and 75 collagen peptides were identified from AAE, AED and PD, respectively. Angiotensin converting enzyme (ACE) inhibitory activity,dipeptidyl peptidase IV(DPP-IV) inhibitory activity,prolyl endopeptidase(PEP) inhibitory activity and antioxidant activity were used to evaluate the bioactivities of AAE,AED and PD,and it was found that the sequence of their bioactivities was AAE
