Extraction of Eight Ginsenosides from Leaves of Panax quinquefolium L. By Ultrasoinc Fountain-based Ultrasonic-assisted Nebulization Extraction Coupled with Solid Phase Extraction

VernacularTitle:基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷
Author: Pei-Xu ZHANG ¹ ; Yong-Ri JIN ; Li CUI ; Qian WU ; Peng LI ; Han-Qi ZHANG ; Xu-Wen LI
Author Information

1. 吉林大学化学学院
Keywords: Ultrasonic-assisted nebulization extraction; Solid phase extraction; Ultrasonic fountain; Panax quinquefolium L.; Ginsenosides
From: Chinese Journal of Analytical Chemistry 2018;46(4):594-600
CountryChina
Language:Chinese
Abstract: A new ultrasonic-assisted extraction (UANE) method coupled with solid phase extraction (SPE) using ultrasonic fountain was established for the extraction of eight common ginsenosides from leaves of Panax quinquefolium L. The extraction system has been designed and several experimental parameters,including the type and volume of extraction solvent,pH value and salt concentration of extraction solvent,type and volume of elution solvent,and amount of C18, extraction time were examined and optimized. Under the optimal conditions,the recoveries of ginsenosides were in the range of 96. 3% -110. 6%, the relative standard deviations (RSDs) were in the range of 2.8%-4.3%,indicating that the method has a good performance for the extraction of these ginsenosides. Compared with traditional UANE-SPE method, the modified method simplified the extraction device,shortened the extraction time and improved the extraction efficiency.