Three Glass Electrospray Ionization Devices for Direct Mass Spectrometry Analysis Using Sharp Slide, Slender Capillary and Sharp Dropper
10.11895/j.issn.0253-3820.171305
- VernacularTitle:使用尖玻片、毛细管和尖滴管三种玻璃尖端电喷雾离子化质谱分析方法
- Author:
Yu-Yu HUANG
1
;
Kun XIAO
;
Xing-Li PEI
;
Yu-Liang HAN
;
Can GONG
;
Xu XU
Author Information
1. 上海应用技术大学 化学与环境工程学院
- Keywords:
Mass spectrometry ionization;
Mass spectrometry injection;
Sharp electrospray;
Glass electrospray
- From:
Chinese Journal of Analytical Chemistry
2018;46(4):578-585
- CountryChina
- Language:Chinese
-
Abstract:
Three kinds of glass sharp electrospray ionization devices were built for direct mass spectrometry (MS) analysis using sharp slide,slender capillary and sharp dropper. Through the simulation experiment and mass spectrometry experimental investigation,the influence of the experimental conditions was studied. Under the optimum working conditions such as the electrospray tip diameter was less than 0.24 mm, the distance between the electrospray tip and the MS sample inlet was 10 mm, the electrospray voltage was 4.5 kV, the electrospray capillary angle was about 5.0°-7.5° (upper at back) and the left and right angles were 0°, 0.1 mg/mL sample solution(containing 1% formic acid for positive ion detection,or ammonium hydroxide for negative ion detection) could be well analyzed by MS. In positive or negative ion modes, the quinine, mandelic acid,benzoin,phenylalanine and other different types of sample solutions were analyzed by MS. The result showed that quinine,mandelic acid,benzoin,phenylalanine,tartaric acid,2,5-dihydroxybenzoic acid (DHB) and tangeretin could be detected in positive ion mode using sharp dropper electrospray. DHB, phenylalanine, tartaric acid and mandelic acid were detected in negative ion mode. Sharp slide electrospray was suitable for measuring the stable sample in the air, capillary electrospray and sharp dropper electrospray were suitable for detecting many low viscosity sample solution even instable sample. Using the method, the extraction solution of propranolol hydrochloride tablet and berberine hydrochloride tablet was directly analyzed by MS. The results showed that the glass sharp electrospray method had the advantages such as without carrier gas, simple structure, easy to build and operate, rapid analysis, anti-cross pollution, and could be conveniently used for MS analysis.
