Monitoring of Oxidation Process of Human Milk Lipid Substitutes by Molecular Fluorescence Spectroscopy
10.11895/j.issn.0253-3820.171230
- VernacularTitle:同步荧光法监控人乳脂质替代品氧化过程
- Author:
Xue LI
1
;
Jun CAO
;
Xin-Peng BAI
;
Fang-Fang ZHANG
Author Information
1. 海南大学食品学院
- Keywords:
Human milk lipid substitute;
Schaal oven accelerated oxidation process;
Oxidation index;
Synchronous fluorescence
- From:
Chinese Journal of Analytical Chemistry
2018;46(4):543-549
- CountryChina
- Language:Chinese
-
Abstract:
The fatty acid contents in soybean oil, corn oil, coconut oil, sunflower seed oil and fish oil were detected by gas chromatography. According to the characteristics of fatty acids of raw oil and the relevant regulations of GB 10765-2010,the formula of 6 kinds of human milk lipid substitutes was designed by Matlab software and Excel linear programming. At the same time, the Schaal oven accelerated oxidation method was used to study the oxidation process of 6 formulations using synchronous fluorescence technique combined with traditional chemical reagents (oxidation index:peroxide value,value of fennel and total oxidation value). By statistical analysis of data, the statistical relationship between fluorescence intensity and oxidation index was explored. The results showed that the main fatty acids of coconut oil were lauric acid and myristic acid,and its saturated fatty acid content was the highest 93.75% ±0.06%;while all the other oils were mainly composed of palmitic acid,linoleic acid and other components,wherein the fish oil had the highest content of unsaturated fatty acid. With the increase of oxidation time,the fluorescence intensity decreased gradually and the oxidation index value increased continuously. The correlation analysis and regression analysis results showed that the change of oxidation index and the fluorescence intensity of 6 samples had negative correlation, and the fluorescence intensity and the peroxide value and total oxidation value was quadratic linear correlation, and anisidine value as a linear correlation had strong correlation.
