Quantitative Determination of Arsenic Species in Feed Using Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry
10.11895/j.issn.0253-3820.171228
- VernacularTitle:液相色谱-氢化物发生原子荧光光谱法测定饲料中砷的形态
- Author:
Cheng-Xin LIU
1
;
Zhi-Ming XIAO
;
Zheng JIA
;
Jing TIAN
;
Xiao-Lu LIU
;
Xia FAN
Author Information
1. 中国农业科学院农业质量标准与检测技术研究所
- Keywords:
Liquid chromatography;
Hydride generation-atomic fluorescence spectrometry;
Feed;
Arsenic;
Species analysis
- From:
Chinese Journal of Analytical Chemistry
2018;46(4):537-542
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive and rapid method using liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS) was developed for the simultaneous determination of seven arsenic species As3+, As5+,MMA, DMA, p-ASA, 4-OH and ROX in feeds. The isolation of the analytes from feed samples was accomplished using methanol water (1:1, V/V). The target compounds were separated on a PRP-X100 anion exchange column and then analyzed by HG-AFS. The mobile phase was 15 mmol/L (NH4)2HPO4and 10 mmol/L potassium acid phthalate. Good linearity was obtained for all of the seven arsenic species, with linear coefficients higher than 0.9964. The LODs of the seven arsenic species were between 5 and 30 μg/kg. Average recoveries for the seven analytes were in the ranges of 76.3%-108.1%, with intra- and inter-day repeatability lower than 7.7% and 17.4%,respectively. This validated method was successively applied to the determination of arsenic species in feed. This method was sensitive,simple,cheap and low operation cost,and could be used for the determination of the arsenicspecies in feeds.
