Electrochemical Immunosensor for Microcystin-LR Detection Based on Nano-composite Material Immobilization and Enzymatic Amplification
10.11895/j.issn.0253-3820.171055
- VernacularTitle:基于双层酶信号放大及纳米功能界面的微囊藻毒素电化学免疫传感器
- Author:
Yan-Yan YIN
1
,
2
;
Li HOU
;
Li-Li ZHANG
;
Xue-Bin LIN
;
Xiao-Ping WU
Author Information
1. 食品安全与生物分析教育部重点实验室(福州大学)
2. 福州大学化学学院
- Keywords:
Horseradish peroxidase;
Multi-walled carbon nanotubes;
Gold nanoparticles;
Microcystin-LR;
Electrochemical immunosensor
- From:
Chinese Journal of Analytical Chemistry
2018;46(4):493-501
- CountryChina
- Language:Chinese
-
Abstract:
A competitive electrochemical immunosensor based on the nano-composite material immobilization and enzymatic amplification was designed for detection of microcystin-LR. Gold nanoparticles/carboxylated multi-walled carbon nanotubes (AuNPs/c-MWCNTs) composite film, which formed by electrodepositing of AuNPs on the C-MWCNTs modified glassy carbon electrode,was used for the immobilization of the antibody of microcystin-LR (anti-MCLR). Horseradish peroxidase (HRP) was introduced onto the nanocomposite interface by HRP blocked sensing interface and specific capture of antibody with target. It could be employed to catalyze the reduction of H2O2, and to block the possible remaining active sites as well. A competitive immunoreaction between antigen and MCLR-HRP was used for target analysis. In the presence of H2O2and hydroquinone (HQ),MCLR could be indirectly detected with differential pulse voltammetry (DPV) method by determining the reduction current of HQ. Under the optimal conditions, the proposed immunosensor exhibited wide linear ranges in the concentration ranges of 0.1-100.0 μg/L, with a detection limit of 0.038 μg/L (S/N = 3,n=8). The immunosensor showed good specificity, stability and sensitivity. It was used to determine MCLR in real water samples with the recoveries of 72.9%-117.3%.
