Determination of 11Kinds of Aminoglycosides in Aquatic Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Molecularly Imprinted Polymers Solid Phase Extraction
10.11895/j.issn.0253-3820.171175
- VernacularTitle:分子印迹聚合物固相萃取-超高效液相色谱-串联质谱法检测水产品中11种氨基糖苷类药物残留
- Author:
Yuan-Fei HUANG
1
;
Xiao-Yi LOU
;
Zhe ZHOU
;
Yang WANG
;
Cong KONG
;
Dong-Mei HUANG
;
You-Qiong CAI
;
Hui-Juan YU
Author Information
1. 中国水产科学研究院东海水产研究所 农业部水产品风险评估实验室上海
- Keywords:
Molecularly imprinted polymers solid phase extraction;
Ultra performance liquid chromatography-tandem mass spectrometric;
Aquatic products;
Aminoglycosides
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):454-461
- CountryChina
- Language:Chinese
-
Abstract:
An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed for the determination of 11 kinds of aminoglycosides (AGs), including paromomycin, spectinomycin, tobramycin, gentamycin, kanamycin, hygromycin B, apramycin, streptomycin, dihydrostreptomycin,amikacin and neomycin in aquatic products. Samples were extracted by phosphate buffer solution, and purified on molecularly imprinted polymers (MIP) solid phase extraction column. After separated by Obelisc R chromatographic column, AGs were detected by UPLC-MS/MS. It showed a good linearity relationship in the AGs concentration range of 1.0-1000 ng/mL with the correlation coefficient R2>0.994. The limit of detection (LOD,S/N≥3) was ranged from 1.0 μg/kg to 10.0 μg/kg,and the limit of quantitation (LOQ,S/N≥10) was ranged from 2.0 μg/kg to 20.0 μg/kg. Besides, the average recoveries presented 78.4%-109.6% with the relative standard deviation (RSD, n=6) of 2.3%-14.9%. This method was successfully applied to the simultaneous determination of 11 kinds of AGs with high sensitivity in aquatic products.
