Study on Tissue Distribution of A Variety of Endogenous Metabolites by Air Flow Assisted Ionization-Ultra High Resolution Mass Spectrometry Imaging
10.11895/j.issn.0253-3820.171348
- VernacularTitle:基于空气动力辅助离子化-超高分辨质谱成像技术的大鼠肾脏组织中多种类代谢物的分布研究
- Author:
Zhong-Hua WANG
1
;
Bing-Shu HE
;
Cheng-Long SUN
;
Xiao-Wei SONG
;
Jiu-Ming HE
;
Rui-Ping ZHANG
;
Zeper ABLIZ
Author Information
1. 中央民族大学生命与环境科学学院
- Keywords:
Air flow assisted ionization;
Mass spectrometric imaging;
Endogenous metabolites;
Kidney
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):406-412
- CountryChina
- Language:Chinese
-
Abstract:
As a promising new molecular imaging technique,mass spectrometry imaging(MSI) has attracted more and more attention in the field of biomedicine. A method of air flow assisted ionization-ultra high resolution mass spectrometry-based mass spectrometric imaging (AFAI-MSI) was developed to profile endogenous metabolites in rat kidney tissue in this study. Rat kidneys were collected and cut into frozen tissue sections,and then were analyzed on an AFAI-MSI system in positive ion mode using acetonitrile-isopmpyl alcohol-water (4:4:2,V/V,5 μL/min) as spray solvent,N2as spray gas(0.6 MPa) and air as assisting gas (45 L/min). The mass range and resolution were set to be 70-1000 Da and 70000, respectively. As a result,a total of 38 metabolites, including organic amines, sugars, vitamins, peptides, neurotransmitters, organic acids,phospholipids,sphingolipids,glyceride,and cholesterol esters, were identified and imaged to characterize their tissue-specific distribution in kidney tissues, and some metabolites, such as choline, acetylcoline,betaine,phoshocholine,and glycerophosphocholine were found to have distinct distribution along the cortex-medulla axis,which may be involved in the formation of osmotic pressure gradient in the kidney. The proposed ultra high resolution mass spectrometry based AFAI-MSI method could work without sample pretreatment, showed high sensitivity and wide metabolite coverage, and was expected to provide a new analytical approach for the research of in situ characterization and metabolic regulation mechanism of endogenous metabolites in kidney.
