In vivo Breath Analysis by Extractive Electrospray Ionization-Mass Spectrometry for Investigation of Metabolic Responses to Traditional Chinese Medicine Massages
10.11895/j.issn.0253-3820.171359
- VernacularTitle:呼出气体活体质谱分析揭示中医推拿引起的代谢反应
- Author:
Teng-Gao ZHU
1
;
Jing HAN
;
Jun-Wen SHU
;
Mu-Fang KE
;
Dan WANG
;
Wen-Jie LIU
;
Nian-Xiang LIN
;
Huan-Wen CHEN
Author Information
1. 东华理工大学
- Keywords:
Exhaled breath;
Mass spectrometry;
Partial least squares discrimination analysis;
Traditional Chinese Medicine massage
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):400-405
- CountryChina
- Language:Chinese
-
Abstract:
Traditional Chinese Medicine massage is a kind of physiotherapy which affects on specific parts of the body surface by means of training to regulate the function of the body to achieve the therapeutic effect. In this work,under positive detection model, the chemical fingerprint of exhaled breath from volunteers before and after receiving Traditional Chinese Medicine massage within m/z 50-1000 were detected by extractive electrospray ionization-mass spectrometry (EESI-MS). And through high resolution mass spectrometry analysis, the metabolites such as epinephrine (m/z 184. 0889), 3-(3-hydroxyphenyl) propionic acid (m/z 167.0615) and L-tryptophan (m/z 205. 0933) were successfully identified. Besides, chemical fingerprints of volunteers before and after receiving Traditional Chinese Medicine massage under different health condition were clearly differentiated via partial least squares discrimination analysis (PLS-DA). The results showed that Traditional Chinese Medicine massage could significantly change the metabolic process of volunteers. Moreover, it further indicated that the established method could provide a real time fashion to follow metabolic changes caused by Traditional Chinese Medicine massage.
