Rapid Characterization of Triacylglycerols in Edible Oils by Matrix-assisted Laser Desorption/Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
10.11895/j.issn.0253-3820.171281
- VernacularTitle:基质辅助激光解吸电离-傅里叶变换离子回旋共振质谱技术用于食用油中甘油三酯的快速分析
- Author:
Wei-Feng LI
1
;
Qiu-Xia YANG
;
Ze-Peng LIN
;
Li-Ping WANG
;
Peng-Ran GUO
Author Information
1. 广东省测试分析研究所
- Keywords:
Matrix-assisted laser desorption/ionization;
Fourier transform ion cyclotron resonance mass spectrometry;
Triacylglycerols;
Principal component analysis
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):393-399
- CountryChina
- Language:Chinese
-
Abstract:
Content and type of triacylglycerols(TAGs) in edible oils are closely related with our health,it is of significance to develop a fast and high-efficiency method for the determination of TAGs. In this manuscript, a fast and direct method for qualitative analysis of TAGs was established using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS). 2,5-DHB was employed as matrix and dichloromethanewas used as solvent for dissolving edible oils. With laser power of 15%,laser frequency of 100 Hz and 100 laser shots, repeatability was evaluated using relative standard deviation (RSD) and less than 10% was obtained. Different kinds of edible oils could be directly distinguished from each other using MS and MS/MS results. With confidence level of 95%, principal component analysis(PCA) results show that 34 different kinds of edible oils were clearly classified. Using this method 5% doped canola in olive was identified directly,indicating that MALDI-FTICR-MS has the potential for rapid analyzing and screening edible oils.
