Preparation of Protein Hybrid Fluorescence Nanoclusters for Rapid Detection of Mercury Ion
10.11895/j.issn.0253-3820.170353
- VernacularTitle:蛋白质杂化荧光金纳米簇的制备及在汞离子快速检测中的应用
- Author:
Tao PENG
1
;
Jian-Yi WANG
;
San-Lei XIE
;
Kai YAO
;
Shu-Juan SUN
;
Yu-Yang ZENG
;
Hai-Yang JIANG
Author Information
1. 中国农业大学动物医学院,北京100193
- Keywords:
Bovine serum albumin;
Fluorescence gold nanoclusters;
Mercury ion;
Tap water
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):373-378
- CountryChina
- Language:Chinese
-
Abstract:
One-step green synthetic approach,with bovine serum albumin(BSA) as stabilizer and reductant, was developed for preparation of BSA hybrid fluorescence gold nanoclusters (AuNCs@BSA). The prepared AuNCs@BSA exhibited strong red fluorescence under UV light illumination. Upon excited at 360 nm, the fluorescence spectrum of AuNCs@ BSA exhibited maximum emission peak at 635 nm. AuNCs@ BSA was presented as uniform spherical morphology with diameter at (2.0 ±0.05) nm. The fluorescence of AuNCs@BSA could be quenched by Hg2+because of its metallophilic reaction. Based on the fluorescent spectrometry, a rapid detection system was developed for Hg2+detection in tap water. The AuNCs@BSA amount, pH and buffer system were optimized in this study. According to optimization results, ultrapure water (pH 5.0) was selected to dilute the AuNCs@BSA by 100 times, and 50 μL/well of AuNCs@BSA dilution was applied to detect mercury ion in tap water. Under the optimized conditions, the detection could be completed within 3 min,the fluorescence intensity of the system was linearly proportional to the concentration of mercury ion in the range of 0.5–900 μg/L with linear equations y=-26.76lgx+803.1(0.5-75 μg/L,R2=0.9951) and y=-0.27x+762.02 (75-900 μg/L,R2=0.9959). The limit of detection was 0.14 μg/L(3σ). The average recoveries in spiked tape water samples ranged from 86.8%-113.4% with relative standard deviation of less than 15%. The result implied that the developed method was able to apply to detect mercury ion rapidly, sensitively and conveniently.
