Chiral Probe for Recognition of Landiolol Hydrochloride and Its Stereoisomer by Mass Spectrometry
10.11895/j.issn.0253-3820.171007
- VernacularTitle:手性酰氯探针对于盐酸兰地洛尔及其立体异构体识别的质谱研究
- Author:
Tian-Xing DAI
1
;
Cai-Yu ZHANG
;
Ying GENG
;
Lan HE
Author Information
1. 中国食品药品检定研究院
- Keywords:
Chiral probe;
Recongnition;
Mass spectrometry;
Landiolol hydrochloride
- From:
Chinese Journal of Analytical Chemistry
2018;46(3):332-341
- CountryChina
- Language:Chinese
-
Abstract:
In this work,two chiral chloride probes were used to differentiate landiolol hydrochloride by mass spectrometry. Two chiral chloride probe reagents, N-(p-Tosyl)-L-phenylalaninyl chloride (TSPC) and (-)-Camphanic acid chloride, were chosen to react with landiolol hydrochloride and its stereoisomers to form covalent bonding derivatives, which enlarged the difference of stereo structure between landiolol and its stereoisomers. Result of tandem mass spectrometry showed that fragment from derivative products prefers to losing water to form fragment ions m/z 793 and m/z 672. The relative abundance of ions m/z 793 and m/z 672 was quite different in each isomer. The fragment ions m/z 603 from (-)-Camphanic acid chloride derivative products showed distinction relative abundance because of the different stability of each stereoisomers, which gave rise to the enlarged difference of stereo structure between landiolol and its stereoisomers. By comparing the different relative abundance ratio of analyte and each stereoisomer in MS/MS spectra, we could realize recognization landiolol hydrochloride and its stereoisomers. Accurate masses of precursor and fragment ions were confirmed on an IT-TOF mass spectrometer. This method by using ion-trap mass spectrometry could rapidly and simply differentiate landiolol hydrochloride and its stereoisomers. This work could also contribute to differentiation and discrimination of landiolol hydrochloride and its stereoisomers.
